Van Leeuwenhoek Centre for Advanced Microscopy

of the Swammerdam Institute for Life Sciences (SILS)
of the Science Faculty (FNWI)
of the University of Amsterdam (UvA)
organizes

PhD Course

Confocal Microscopy

Fundamentals, Advanced techniques and Biological Applications

May 29th - June 1st, 2012

The course "Confocal Light Microscopy" is an advanced course for PhD students and technicians in biology and biomedical science who need confocal microscopy and other light microscopy technique for their experiments. It is our aim to teach how advanced light microscopy techniques can be used in biological research.

After this course, students will understand the basic optical principles of the confocal microscope and its limitations due to the laws of physics. In this course we will focus on basic technologies that can be used in biology such as multi-colour confocal microscopy, the use of co-localization coefficients, phototoxicity and how to keep cells alive under the microscope. Furthermore, students will be introduced in the latest developments in advanced technologies like measurement of molecular interaction (FRET, FLIM), of molecular mobility (FRAP, FLIP), live-cell imaging (CLEM, spinning disk), high resolution imaging techniques (e.g. PALM, TIRF), spectral imaging and image analysis.

The course consists of theoretical lectures (50%) and hands-on experiments (50%). The faculty consists of experts in the field of light microscopy from the Centre of Advanced Microscopy and other universities. Students are encouraged bring their own samples and to discuss their specific problems with the faculty.

For more info see below or download the pdf here (will be added soon).

Topics:















 

- Confocal principle and the effect of the pinhole

- Geometrical optics, optical waves and diffraction

- Aberrations, magnification and resolution

- Laser and detectors

- Fluorescence and fluorescent probes

- Nyquist sampling theorem

- Image deconvolution and restoration

- Colocalisation measurement and image analysis

- Live cell microscopy, Controlled Light Exposure Microscopy (CLEM)

- Measurement of FRET, Fluorescence Lifetime Imaging (FLIM)

- Measurement of molecular mobility (FRAP, FLIP)

- High resolution microscopy (TIRF, PALM, STED)

 
General supervision:
 

Dr. E.M.M. Manders

Speakers:




 

Prof. Dr T.W.J. Gadella (Univ. Amsterdam),

Dr M. Hink (Univ. Amsterdam),

Dr J. Goedhart (Univ. Amsterdam),

Dr A. Houtsmuller (Erasmus Univ. Rotterdam),                                            

Dr R. Hoebe (Academic Medical Centre, Amsterdam),

Dr. H. van der Voort (Scientific Volume Imaging BV),

Ir. R. Breedijk (Univ. Amsterdam), 

Prof. dr. H. Gerritsen (Utrecht University),

Prof. dr. G.J. Brakenhoff (Univ. Amsterdam),

Dr. E. Manders (Univ. Amsterdam)

Credits:

      2 EC

Course fee:

      EUR 750,- (reduced fee for SILS students). The course fee includes the course book and lunches.

Registration: 

     If you're interested to participate in this course, please register the form here (will be added soon). 

     We will make a selection of 32 students and inform you about your acceptation to the course on 
     May 7th, 2012. After your confirmation, we will send you an invoice for the course fee.

Location:

     van Leeuwenhoek Centre for Advanced Microscopy,
     Swammerdam Institute for Life Sciences,
     University of Amsterdam
     Science Park 904 (Room C2.268),
     1098 XH Amsterdam
      (click here)

Information:

      Dr. Erik Manders,
      email: confocal.amsterdam@Gmail.com
      phone: +31 (0)20 - 525 6225