Single-molecule microscopy for cell biology

Prof.dr. T. Schmidt
Leiden Institute of Physics (LION)
University Leiden

A multitude of signal transduction cascades are initiated by interactions between proteins at the plasma membrane resulting in complex cellular responses. To fully understand the different steps in signal transduction processes it is important to study the initial steps of the signaling cascade. We focus on the development of a single-molecule microscopy technique to study processes at the level of individual proteins and identifying the most suited markers for single-molecule in vivo experiments.